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Diabetes mellitus new research

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    Modeling long-term diabetes and related complications in rats J Pharmacol Toxicol Methods.

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    Mar-Apr ; Diabetes mellitus new research Nov Gyömrői út Electronic address: erika. DOI: The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the pathological changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia.

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    Methods: Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control Diabetic groups. Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests. Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electron microscopy.

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    Discussion: We established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period.

    Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical trials. Our results provide a useful basis for designing studies for testing preventative treatments as well as other translational medical research in this field.

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    Keywords: Anterior segment neovascularization; Chronic model; Cold allodynia; Diabetic nephropathy; Diabetic neuropathy; Diabetic retinopathy; Drug development; Insulin implant; Preclinical model; Streptozotocin. Copyright © Youtube cukorbetegség Inc. All rights reserved.

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